Molecular Characteristics
Complete Specifications
Structural Composition
D-retro-inverso analog of the FOXO4 p53-interaction domain peptide sequence
Physical Properties
FOXO4-DRI is a synthetic peptide engineered using a D-retro-inverso configuration, meaning the amino acid sequence is reversed and composed of D-amino acids rather than the naturally occurring L-forms. This structural modification enhances resistance to enzymatic degradation and increases stability under controlled research conditions. The peptide’s modified backbone contributes to improved structural persistence compared to conventional L-peptides. Lyophilized storage and protection from moisture, light, and repeated freeze–thaw cycles help maintain analytical consistency for laboratory applications.
Analysis & Documentation
Identity
Each production lot is checked against the reference structure recorded for this compound:
- Classification — Synthetic D-retro-inverso peptide
- Molecular formula — C₁₅₈H₂₅₆N₄₆O₄₄
- Expected mass — ~3,545.1 Da
- CAS registry — Not assigned (research peptide)
- Chain length — 13 amino acids (D-retro-inverso configuration)
- PubChem CID — Not available
Analytical Methods
Testing is carried out per lot by an independent laboratory. The methods measure what the material is and how much of it is the target compound:
- RP-HPLC — reverse-phase separation of the target from process-related impurities; purity is reported as area percent of the main peak
- Mass spectrometry — observed mass compared against the expected molecular mass above
- Retention time — compared against a reference standard run under the same conditions
- Chromatogram — reproduced on the certificate so the peak and any shoulders can be inspected directly
Material & Documentation
What arrives, and how to tie it back to its paperwork:
- Appearance as supplied — White to off-white lyophilized powder
- Solubility — Water, phosphate-buffered saline (PBS)
- Lot number — printed on the container label and used to look up the certificate
- Certificate — indexed by lot at Certificates of Analysis
Laboratory Handling and Storage Protocols
Lyophilized Powder Storage
- Store at –20°C to –80°C in the original, sealed vial
- Protect from light exposure and moisture
- A desiccated storage environment is recommended
- Stability data suggests extended stability when stored at −20 °C or below.
Reconstituted Solution Storage
- Short-term storage: Up to 7 days at 4°C
- Long-term storage: Store at –20°C in aliquots
- Use single-use aliquots to preserve peptide integrity
- Minimize freeze–thaw cycles; single-use aliquots are strongly recommended
Stability Characteristics
FOXO4-DRI is a synthetic peptide research compound that demonstrates stable handling characteristics when managed according to standard peptide laboratory protocols. Proper cold storage of the lyophilized material, careful reconstitution, and minimized freeze–thaw exposure help preserve structural integrity and solubility. When handled appropriately, FOXO4-DRI supports consistent use in in vitro and analytical research applications.
Frequently Asked Questions
What is FOXO4-DRI?
FOXO4-DRI is a modified peptide engineered to disrupt the FOXO4–p53 protein interaction in experimental research models. It is commonly used as a tool compound in senescence and cellular aging studies.
What does “DRI” mean?
“DRI” stands for D-retro-inverso, a structural modification in which amino acids are reversed and synthesized using D-enantiomers. This design increases peptide stability and resistance to enzymatic degradation in research models.
Is FOXO4-DRI a naturally occurring peptide?
No. FOXO4-DRI is a synthetically engineered peptide designed specifically for research purposes and does not occur naturally in biological systems.
How is FOXO4-DRI supplied?
FOXO4-DRI is typically supplied as a lyophilized (freeze-dried) powder in sealed research vials to maintain stability during storage and shipment.
How should unreconstituted FOXO4-DRI be stored?
Lyophilized FOXO4-DRI should be stored:
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Long-term: −20 °C to −80 °C
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Short-term: 2–8 °C
Keep vials sealed and protected from light and moisture until use.
How should reconstituted FOXO4-DRI be handled and stored?
Once reconstituted:
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Store at 2–8 °C for short-term laboratory use
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For extended storage, aliquot and freeze at −20 °C or below
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Minimize freeze–thaw cycles using single-use aliquots
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Gently swirl to mix; avoid vigorous agitation
Is FOXO4-DRI FDA-approved?
No. FOXO4-DRI is not FDA-approved as a drug, supplement, or therapeutic product. It is sold exclusively as a research compound and must not be marketed or used for diagnostic, therapeutic, or consumption purposes.
Does FOXO4-DRI require a Certificate of Analysis (COA)?
Yes. A Certificate of Analysis (COA) should be available for each batch, verifying identity, purity, and analytical testing results to ensure research quality and traceability.
This product is not for human consumption. It is sold strictly for research and educational purposes and is not intended to diagnose, treat, cure, or prevent any disease.
Any clinical data or research information referenced on this page is derived from peer-reviewed scientific literature and official publications. This information is provided for educational reference only and does not constitute medical advice or product claims.
By purchasing this product, you acknowledge that you are a qualified researcher and agree to use it in full compliance with all applicable laws and regulations.
